Journal: Brain Pathology
Article Title: MIF Receptor CD 74 is Restricted to Microglia/Macrophages, Associated with a M 1‐Polarized Immune Milieu and Prolonged Patient Survival in Gliomas
doi: 10.1111/bpa.12194
Figure Lengend Snippet: CD74 mRNA and protein levels correlate with the content of glioma‐associated microglia/macrophages (GAMs). Hierarchical cluster analyses (A) of gene expression signatures of selected key factors of immune cells, glioblastoma cells as well as glioblastoma micromilieu of 424 primary glioblastomas and 11 normal brain samples deriving from the cancer genome atlas (TCGA) data portal using the Agilent 244K G4502A microarray to determine mRNA profiles. Hierarchical clustering of this data was performed using the Ward's minimum variance method. Dark red color indicates a perfect positive correlation (+1) gradually decreasing to a perfect negative correlation (−1) indicated in blue. Correlation analyses of the same data subset comparing CD74 with either (B) Iba1 (AIF1), (C) CD68 or (D) CD11b (ITGAM) are depicted. CD74 and Iba1 immunoblotting (E) of GAMs, glioblastoma cells (PC) and whole glioblastoma tissue (T) of three different patients with primary glioblastoma World Health Organization (WHO) grade IV showing a positive correlation of CD74 and Iba1 protein expression. Actin served as positive control.
Article Snippet: The cancer genome atlas ( TCGA ), the R epository of M olecular B rain N eoplasia D ata ( REMBRANDT ) and R 2: microarray analysis and visualization platform analyses To determine gene expression of the MIF‐CD74 system in gliomas, we additionally analyzed data from the TCGA using the Agilent 244K G4502A microarray to determine mRNA profiles ( http://tcga.cancer.gov/ ; accessed November 2011).
Techniques: Expressing, Microarray, Western Blot, Positive Control